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colonic ex vivo biopsies  (Thermo Fisher)


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    Structured Review

    Thermo Fisher colonic ex vivo biopsies
    <t>Colonic</t> secretion of pro- and anti-inflammatory mediators in Campylobacter infected conventional mice. Conventionally colonized C57BL/6 mice were perorally infected with C. jejuni (open circles) or C. coli (closed circles) on day (d) 0 and d1 by gavage. On day 21 post-infection, (A) IFN-γ, (B) TNF-α, (C) MCP-1, (D) IL-6, (E) IL-12p70 and (F) IL-10 concentrations were determined in supernatants derived from colonic ex <t>vivo</t> <t>biopsies.</t> Naive mice served as negative control animals (open diamonds). Medians (black bars), levels of significance (P -values) assessed by the Kruskal-Wallis test and Dunn’s post-correction and numbers of analyzed animals (in parentheses) are indicated. Data were pooled from four independent experiments
    Colonic Ex Vivo Biopsies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/colonic+ex+vivo+biopsies/PHOSPHATE+BUFFERED+SALINE+PBS/pmc07391380-48-0-13
    Average 99 stars, based on 1 article reviews
    colonic ex vivo biopsies - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "The conundrum of colonization resistance against Campylobacter reloaded: The gut microbota composition in conventional mice does not prevent from Campylobacter coli infection"

    Article Title: The conundrum of colonization resistance against Campylobacter reloaded: The gut microbota composition in conventional mice does not prevent from Campylobacter coli infection

    Journal: European Journal of Microbiology & Immunology

    doi: 10.1556/1886.2020.00004

    Colonic secretion of pro- and anti-inflammatory mediators in Campylobacter infected conventional mice. Conventionally colonized C57BL/6 mice were perorally infected with C. jejuni (open circles) or C. coli (closed circles) on day (d) 0 and d1 by gavage. On day 21 post-infection, (A) IFN-γ, (B) TNF-α, (C) MCP-1, (D) IL-6, (E) IL-12p70 and (F) IL-10 concentrations were determined in supernatants derived from colonic ex vivo biopsies. Naive mice served as negative control animals (open diamonds). Medians (black bars), levels of significance (P -values) assessed by the Kruskal-Wallis test and Dunn’s post-correction and numbers of analyzed animals (in parentheses) are indicated. Data were pooled from four independent experiments
    Figure Legend Snippet: Colonic secretion of pro- and anti-inflammatory mediators in Campylobacter infected conventional mice. Conventionally colonized C57BL/6 mice were perorally infected with C. jejuni (open circles) or C. coli (closed circles) on day (d) 0 and d1 by gavage. On day 21 post-infection, (A) IFN-γ, (B) TNF-α, (C) MCP-1, (D) IL-6, (E) IL-12p70 and (F) IL-10 concentrations were determined in supernatants derived from colonic ex vivo biopsies. Naive mice served as negative control animals (open diamonds). Medians (black bars), levels of significance (P -values) assessed by the Kruskal-Wallis test and Dunn’s post-correction and numbers of analyzed animals (in parentheses) are indicated. Data were pooled from four independent experiments

    Techniques Used: Infection, Derivative Assay, Ex Vivo, Negative Control

    Related Articles

    Ex Vivo:

    Article Title: Vitamin D in Acute Campylobacteriosis–Results From an Intervention Study Applying a Clinical Campylobacter jejuni Induced Enterocolitis Model
    Article Snippet: .. Colonic ex vivo biopsies were cut longitudinally, washed in phosphate buffered saline (PBS; Gibco, Life Technologies, UK), and strips of ~1 cm 2 tissue and ex vivo biopsies derived from MLN (3–4 lymph nodes), liver, and spleen (one half) were placed in 24-flat-bottom well-culture plates (Nunc, Germany) containing 500 μL serum-free RPMI 1640 medium (Gibco, life technologies, UK) supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL; PAA Laboratories, Germany). .. After 18 h at 37°C, respective culture supernatants as well as serum samples were tested for IL-6, monocyte chemoattractant protein 1 (MCP-1), tumor necrosis factor (TNF), and interferon-γ (IFN-γ) by the Mouse Inflammation Cytometric Bead Assay (CBA; BD Biosciences, Germany) on a BD FACSCanto II flow cytometer (BD Biosciences).

    Article Title: The conundrum of colonization resistance against Campylobacter reloaded: The gut microbota composition in conventional mice does not prevent from Campylobacter coli infection
    Article Snippet: .. Colonic ex vivo biopsies were cut longitudinally, washed in phosphate buffered saline (PBS; Gibco, Life Technologies, UK), and strips of approximately 1 cm 2 tissue and ex vivo biopsies derived from the spleen (one half) were placed in 24-flat-bottom well culture plates (Nunc, Germany) containing 500 μL serum-free RPMI 1640 medium (Gibco, life technologies, UK) supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL; PAA Laboratories, Germany). .. After 18 h at 37 °C, respective culture supernatants, as well as serum samples were tested for interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein 1 (MCP-1), and interleukin (IL) -6, IL-12p70 and IL-10 by the Mouse Inflammation Cytometric Bead Assay (CBA; BD Biosciences, Germany) on a BD FACSCanto II flow cytometer (BD Biosciences).

    Saline:

    Article Title: Vitamin D in Acute Campylobacteriosis–Results From an Intervention Study Applying a Clinical Campylobacter jejuni Induced Enterocolitis Model
    Article Snippet: .. Colonic ex vivo biopsies were cut longitudinally, washed in phosphate buffered saline (PBS; Gibco, Life Technologies, UK), and strips of ~1 cm 2 tissue and ex vivo biopsies derived from MLN (3–4 lymph nodes), liver, and spleen (one half) were placed in 24-flat-bottom well-culture plates (Nunc, Germany) containing 500 μL serum-free RPMI 1640 medium (Gibco, life technologies, UK) supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL; PAA Laboratories, Germany). .. After 18 h at 37°C, respective culture supernatants as well as serum samples were tested for IL-6, monocyte chemoattractant protein 1 (MCP-1), tumor necrosis factor (TNF), and interferon-γ (IFN-γ) by the Mouse Inflammation Cytometric Bead Assay (CBA; BD Biosciences, Germany) on a BD FACSCanto II flow cytometer (BD Biosciences).

    Article Title: The conundrum of colonization resistance against Campylobacter reloaded: The gut microbota composition in conventional mice does not prevent from Campylobacter coli infection
    Article Snippet: .. Colonic ex vivo biopsies were cut longitudinally, washed in phosphate buffered saline (PBS; Gibco, Life Technologies, UK), and strips of approximately 1 cm 2 tissue and ex vivo biopsies derived from the spleen (one half) were placed in 24-flat-bottom well culture plates (Nunc, Germany) containing 500 μL serum-free RPMI 1640 medium (Gibco, life technologies, UK) supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL; PAA Laboratories, Germany). .. After 18 h at 37 °C, respective culture supernatants, as well as serum samples were tested for interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein 1 (MCP-1), and interleukin (IL) -6, IL-12p70 and IL-10 by the Mouse Inflammation Cytometric Bead Assay (CBA; BD Biosciences, Germany) on a BD FACSCanto II flow cytometer (BD Biosciences).

    Derivative Assay:

    Article Title: Vitamin D in Acute Campylobacteriosis–Results From an Intervention Study Applying a Clinical Campylobacter jejuni Induced Enterocolitis Model
    Article Snippet: .. Colonic ex vivo biopsies were cut longitudinally, washed in phosphate buffered saline (PBS; Gibco, Life Technologies, UK), and strips of ~1 cm 2 tissue and ex vivo biopsies derived from MLN (3–4 lymph nodes), liver, and spleen (one half) were placed in 24-flat-bottom well-culture plates (Nunc, Germany) containing 500 μL serum-free RPMI 1640 medium (Gibco, life technologies, UK) supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL; PAA Laboratories, Germany). .. After 18 h at 37°C, respective culture supernatants as well as serum samples were tested for IL-6, monocyte chemoattractant protein 1 (MCP-1), tumor necrosis factor (TNF), and interferon-γ (IFN-γ) by the Mouse Inflammation Cytometric Bead Assay (CBA; BD Biosciences, Germany) on a BD FACSCanto II flow cytometer (BD Biosciences).

    Article Title: The conundrum of colonization resistance against Campylobacter reloaded: The gut microbota composition in conventional mice does not prevent from Campylobacter coli infection
    Article Snippet: .. Colonic ex vivo biopsies were cut longitudinally, washed in phosphate buffered saline (PBS; Gibco, Life Technologies, UK), and strips of approximately 1 cm 2 tissue and ex vivo biopsies derived from the spleen (one half) were placed in 24-flat-bottom well culture plates (Nunc, Germany) containing 500 μL serum-free RPMI 1640 medium (Gibco, life technologies, UK) supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL; PAA Laboratories, Germany). .. After 18 h at 37 °C, respective culture supernatants, as well as serum samples were tested for interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein 1 (MCP-1), and interleukin (IL) -6, IL-12p70 and IL-10 by the Mouse Inflammation Cytometric Bead Assay (CBA; BD Biosciences, Germany) on a BD FACSCanto II flow cytometer (BD Biosciences).



    Similar Products

    colonic ex vivo biopsies  (Thermo Fisher)
    99
    Thermo Fisher colonic ex vivo biopsies
    <t>Colonic</t> secretion of pro- and anti-inflammatory mediators in Campylobacter infected conventional mice. Conventionally colonized C57BL/6 mice were perorally infected with C. jejuni (open circles) or C. coli (closed circles) on day (d) 0 and d1 by gavage. On day 21 post-infection, (A) IFN-γ, (B) TNF-α, (C) MCP-1, (D) IL-6, (E) IL-12p70 and (F) IL-10 concentrations were determined in supernatants derived from colonic ex <t>vivo</t> <t>biopsies.</t> Naive mice served as negative control animals (open diamonds). Medians (black bars), levels of significance (P -values) assessed by the Kruskal-Wallis test and Dunn’s post-correction and numbers of analyzed animals (in parentheses) are indicated. Data were pooled from four independent experiments
    Colonic Ex Vivo Biopsies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/colonic+ex+vivo+biopsies/PHOSPHATE+BUFFERED+SALINE+PBS/pmc07391380-48-0-13
    Average 99 stars, based on 1 article reviews
    colonic ex vivo biopsies - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Colonic secretion of pro- and anti-inflammatory mediators in Campylobacter infected conventional mice. Conventionally colonized C57BL/6 mice were perorally infected with C. jejuni (open circles) or C. coli (closed circles) on day (d) 0 and d1 by gavage. On day 21 post-infection, (A) IFN-γ, (B) TNF-α, (C) MCP-1, (D) IL-6, (E) IL-12p70 and (F) IL-10 concentrations were determined in supernatants derived from colonic ex vivo biopsies. Naive mice served as negative control animals (open diamonds). Medians (black bars), levels of significance (P -values) assessed by the Kruskal-Wallis test and Dunn’s post-correction and numbers of analyzed animals (in parentheses) are indicated. Data were pooled from four independent experiments

    Journal: European Journal of Microbiology & Immunology

    Article Title: The conundrum of colonization resistance against Campylobacter reloaded: The gut microbota composition in conventional mice does not prevent from Campylobacter coli infection

    doi: 10.1556/1886.2020.00004

    Figure Lengend Snippet: Colonic secretion of pro- and anti-inflammatory mediators in Campylobacter infected conventional mice. Conventionally colonized C57BL/6 mice were perorally infected with C. jejuni (open circles) or C. coli (closed circles) on day (d) 0 and d1 by gavage. On day 21 post-infection, (A) IFN-γ, (B) TNF-α, (C) MCP-1, (D) IL-6, (E) IL-12p70 and (F) IL-10 concentrations were determined in supernatants derived from colonic ex vivo biopsies. Naive mice served as negative control animals (open diamonds). Medians (black bars), levels of significance (P -values) assessed by the Kruskal-Wallis test and Dunn’s post-correction and numbers of analyzed animals (in parentheses) are indicated. Data were pooled from four independent experiments

    Article Snippet: Colonic ex vivo biopsies were cut longitudinally, washed in phosphate buffered saline (PBS; Gibco, Life Technologies, UK), and strips of approximately 1 cm 2 tissue and ex vivo biopsies derived from the spleen (one half) were placed in 24-flat-bottom well culture plates (Nunc, Germany) containing 500 μL serum-free RPMI 1640 medium (Gibco, life technologies, UK) supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL; PAA Laboratories, Germany).

    Techniques: Infection, Derivative Assay, Ex Vivo, Negative Control